Heterogeneity in glutamic acid decarboxylase expression among single rat pancreatic beta cells
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چکیده
منابع مشابه
Pattern of expression of glutamic acid decarboxylase mRNA in the developing rat brain.
The time and pattern of appearance of glutamic acid decarboxylase (glutamate decarboxylase; EC 4.1.1.15) (GAD) mRNA during the development of the rat brain were analyzed. RNA transfer blot analysis of poly(A)+ RNA from whole brain shows that a 3.7-kilobase transcript is the most abundant form of the message from embryonic day 15 (E15) through adulthood. By E15 this form is present at about 50% ...
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Glutamate decarboxylase 65 (GAD65) produces gamma-aminobutyric acid, the main inhibitory neurotransmitter in adult mammalian brain. Previous experiments, performed in brain, showed that GAD65 gene possesses two TATA-less promoters, although the significance is unknown. Here, by rapid amplification of cDNA ends method, two distinct GAD65 mRNA isoforms transcribed from two independent clusters of...
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1. The metabolism of 131I-labelled thyroglobulin from four mammalian species has been studied. 2. The rat, dog and monkey rapidly metabolize the homologous protein, with the formation of iodide, iodotyrosines and thyroxine. Rabbit thyroglobulin disappears slowly from the plasma of the rabbit after intravenous injection, and no metabolites have so far been detected in the blood. 3. The developme...
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Antibodies directed against the enzyme glutamic acid decarboxylase (GAD) are believed to be the main cause of destruction of pancreatic islet cells in type I (insulin dependent) diabetes mellitus. The enzyme was found both in the brain and pancreatic beta cells. Although similarities in identity of GAD in human and rat brain have been demonstrated, little is known about the interaction betw...
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Background: Autoimmune type 1 diabetes mellitus is caused by T-cell mediated immune destruction of the insulin-producing β-cell in pancreatic islets of Langerhans. Specificity of the auto-antibodies and of the auto-reactive T-cells has been investigated, in which several auto-antigens were proposed. Objective: To determine whether glutamic acid decarboxylase (GAD) feeding would induce oral tol...
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ژورنال
عنوان ژورنال: Diabetologia
سال: 1999
ISSN: 0012-186X,1432-0428
DOI: 10.1007/s001250051275